Dry Column
Chromatography (DCC)
Dry column
chromatography (DCC) is a versatile Prep LC
method. Basically, any sample that can be
separated on silica gel or neutral alumina TLC
plate can also be separated by the corresponding DCC-setup. The dry-column procedure has been
successfully applied for the preparation of
dye-stuffs, alkaloids, and other heterocyclic
substances which are known to be separated on
other types of columns, but, with considerable
difficulties. Lipids have also been successfully
separated.
Dry column chromatography bridges the gap
between analytical TLC and preparative classical
column chromatography. The cost is much less
than the cost incurred for instrumental pressure
associated with preparative liquid
chromatography.
Flash Column
Chromatography (FCC)
Flash
chromatography offers an affordable, simple,
fast, and convenient solution for the
purification of synthetic and natural organic
compounds from crude mixtures. It plays a
critical role in the purification of natural
plant alkaloids. Due to modularity of design and
ease of use flash has become the scale-up
process in the purification of products
initially isolated using thin-layer
chromatography (TLC) chemistry. Prepackaged
flash columns are offered with more than 30
different phase chemistries having corresponding
TLC plates. Using either isocratic or gradient
elution techniques with flash chromatography it
is possible to separate out a desired compound(s)
on a single run.
For more
information about flash, dry column and other
forms of column chromatography, download our
free 75
page primer
here
(one-time
registration required).
The table of
contents appears below.
| 1. Column
Chromatography using DAI Adsorbents |
2.
Chromatography, a Physical Chemical
Separation Technique |
| 3. Column
Chromatography, a Specific
Chromatographic Setup |
4. The
Retention |
| 5. The
Elution |
6. The
Chromatographic Apparatus |
| 7. The
Chromatographic Process |
8. The
Stationary Phase |
|
9.
Substances with and without Retention |
10. The
Peak |
| 11. The
Chromatogram |
12. The
Chromatogram |
|
13.
Measuring Retention and Resolution |
14. Length
of Column |
| 15. Flow
Rate of the Mobile Phase |
16. The
Specificity of the Sorbent |
| 17. The
Particle Diameter of the Sorbent |
18.
Sorbents with Activity and with Constant Activity |
|
19.
Sorbents with Activity and with Constant Activity |
20. The
Temperature |
| 21. The
Polarity of the Solvent |
22.
Deactivation and Constant Deactivation
Behavior |
| 23. The
Capacity |
24. Highly
Standardized Aluminum Oxides for
Chromatography |
| 25. Highly
Standardized Silica Gels for
Chromatography |
26. Notes
Regarding Applications |
|
Examples of the
Technology |
| 1. The
separation of crystal violet from uranium
as an example for demonstrating the
difference between basic and acid
alumina and the influence of the eluent. |
2.
Separation of cis- and trans-azobene on
silica gel plates |
| 3.
Liquid Chromatographic separation of
benzene, amidopyrin and antipyrin |
4. “Dry-Column Chromatography”: A preparative Chromatographic technique with
the resolution power of Thin-Layer
Chromatography |
| 5. The
separation of ferrocene, acetyl
ferrocene and diacetyl ferrocene: A
dry-column experiment |
6.
Thin-Layer Chromatography - Dry Column
Chromatography |
| 7.
Pre-separation of essential oils and
similar complex substance mixtures for
GC-analysis by means of modified
Dry-Column Chromatography |
8.
Analysis of the essential oil of Ocimum
gratissimum L. |
| 9
.Studies on acetylene compounds |
10. A
short contribution to the assay of
aflatoxins |
| 11.
The determination of griseofulvin using
potassium dichromate dissolved in
sulfuric acid |
12. An
exact and rapid method for determining
polycyclic hydrocarbons |
|
13.
Chromatographic determination of sodium
and potassium hydroxide contents of
electroplating baths |
14.
Reactions of toluene sulphonates on an
alumina surface |
|
15. A
sulfur analog of the croconate dianion |
16.
Metal complexes of tetrapyrrol ligands |
| 17.
C-17-oxidation of clavin alkaloids
having primary alcohol hydroxy moieties |
18.
The separation of cyclic nucleoside
monophosphates from other nucleotides
using alumina columns |
| 19. A
reliable method for the determination of
estriol in pregnant urine |
20.
The human gonadotropin cycle |
|
21.Chromatographic separation of
phospholipids using alumina and solvents
containing ammonium salts |
22.Adsorption elution chromatography of
alkylbenzenes using alumina columns |
| 23.An
assay for polycyclic aromatics contained
in Vaseline |
24.Sampling and analytical procedure for
polycyclic aromatics contained in
exhausts |
| 25.The
chromatographic separation of
(carcinogenic) polycyclic aromatics |
|
| |
|
|
Literature References |
Literature of Interest |
|
Journals |
Quick
Reference to all DAI Adsorbents |
|
Adsorbents for Column Chromatography |
Quick
Reference to all DAI Adsorbents TLC
plates |
| The
authors recommend the use of DAI Alumina
and Silica adsorbents which are
manufactured to the specifications
detailed in the following pages. |
|
