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Chromatography
 

Column chromatography flash drycolumn chromatography primer cover

Download FREE 75 Page Primer on Column Chromatography
by Dynamic Adsorbents, Inc.


Dynamic Adsorbents' alumina and silica gel are manufactured and designed to be your best source for adsorbents and material for all types of column chromatography.

Our 75 page primer on column chromatography is  supplemented with timely and germane feature articles of special interest to the pharmaceutical, biotechnology, cosmetic, fragrance, environmental and nutraceutical industries.

See table of contents at the bottom

Click Here to Download Primer

 

 

Dry column chromatographyDry column chromatography
Dry Column Chromatography (DCC)

Dry column chromatography (DCC) is a versatile Prep LC method. Basically, any sample that can be separated on silica gel or neutral alumina TLC plate can also be separated by the corresponding DCC-setup. The dry-column procedure has been successfully applied for the preparation of dye-stuffs, alkaloids, and other heterocyclic substances which are known to be separated on other types of columns, but, with considerable difficulties. Lipids have also been successfully separated.

Dry column chromatography bridges the gap between analytical TLC and preparative classical column chromatography. The cost is much less than the cost incurred for instrumental pressure associated with preparative liquid chromatography.

 

 

Flash Column Chromatography (FCC)

Dynaflash flash column chromatographyFlash chromatography offers an affordable, simple, fast, and convenient solution for the purification of synthetic and natural organic compounds from crude mixtures. It plays a critical role in the purification of natural plant alkaloids. Due to modularity of design and ease of use flash has become the scale-up process in the purification of products initially isolated using thin-layer chromatography (TLC) chemistry. Prepackaged flash columns are offered with more than 30 different phase chemistries having corresponding TLC plates. Using either isocratic or gradient elution techniques with flash chromatography it is possible to separate out a desired compound(s) on a single run.

For more information about flash, dry column and other forms of column chromatography, download our free 75 page primer (one-time registration required).

The table of contents appears below.

1. Column Chromatography using DAI Adsorbents 2. Chromatography, a Physical Chemical Separation Technique
3. Column Chromatography, a Specific Chromatographic Setup 4. The Retention
5. The Elution 6. The Chromatographic Apparatus
7. The Chromatographic Process 8. The Stationary Phase
9. Substances with and without Retention 10. The Peak
11. The Chromatogram 12. The Chromatogram
13. Measuring Retention and Resolution 14. Length of Column
15. Flow Rate of the Mobile Phase 16. The Specificity of the Sorbent
17. The Particle Diameter of the Sorbent 18. Sorbents with Activity and with Constant Activity
19. Sorbents with Activity and with Constant Activity 20. The Temperature
21. The Polarity of the Solvent 22. Deactivation and Constant Deactivation Behavior
23. The Capacity 24. Highly Standardized Aluminum Oxides for Chromatography
25. Highly Standardized Silica Gels for Chromatography 26. Notes Regarding Applications
Examples of the Technology
1. The separation of crystal violet from uranium as an example for demonstrating the difference between basic and acid alumina and the influence of the eluent. 2. Separation of cis- and trans-azobene on silica gel plates
3. Liquid Chromatographic separation of benzene, amidopyrin and antipyrin 4. “Dry-Column Chromatography”: A preparative Chromatographic technique with the resolution power of Thin-Layer Chromatography
5. The separation of ferrocene, acetyl ferrocene and diacetyl ferrocene: A dry-column experiment 6. Thin-Layer Chromatography - Dry Column Chromatography
7. Pre-separation of essential oils and similar complex substance mixtures for GC-analysis by means of modified Dry-Column Chromatography 8. Analysis of the essential oil of Ocimum gratissimum L.
9 .Studies on acetylene compounds 10. A short contribution to the assay of aflatoxins
11. The determination of griseofulvin using potassium dichromate dissolved in sulfuric acid 12. An exact and rapid method for determining polycyclic hydrocarbons

13. Chromatographic determination of sodium and potassium hydroxide contents of electroplating baths

14. Reactions of toluene sulphonates on an alumina surface

15. A sulfur analog of the croconate dianion

16. Metal complexes of tetrapyrrol ligands
17. C-17-oxidation of clavin alkaloids having primary alcohol hydroxy moieties 18. The separation of cyclic nucleoside monophosphates from other nucleotides using alumina columns
19. A reliable method for the determination of estriol in pregnant urine 20. The human gonadotropin cycle
21.Chromatographic separation of phospholipids using alumina and solvents containing ammonium salts 22.Adsorption elution chromatography of alkylbenzenes using alumina columns
23.An assay for polycyclic aromatics contained in Vaseline 24.Sampling and analytical procedure for polycyclic aromatics contained in exhausts
25.The chromatographic separation of (carcinogenic) polycyclic aromatics  
   
Literature References Literature of Interest
Journals Quick Reference to all DAI Adsorbents
Adsorbents for Column Chromatography Quick Reference to all DAI Adsorbents TLC plates
The authors recommend the use of DAI Alumina and Silica adsorbents which are manufactured to the specifications detailed in the following pages.  


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