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Applications

Analgesics, Pharmaceutical Formulations

Isopropylphenazone, Ortho Ethoxybenzamide Caffeine, Mandelic Acid Benzyl Ester - Alumina

 

Operating Conditions

Sample Dissolved in: iso-octane/methylene chloride
Adsorbent Used: DAI Alumina
Stationary Phase: acetic acid 2%
Eluent: iso-octane: methylene chloride = 95.5
Sample Volume: 0.2 ml
Flow rate: 10 ml/min
Operating Pressure: 28 psi
Column Size: 9mm x 300 mm
Temperature: 20ºC
Detection: UV detector 251 nm
Substances Separated Sample (mg) Retention Volume (ml)
1. isopropyl phenazone 0.05 35
2. o-ethoxybenzamide 0.30 55
3. caffeine 0.24 115
4. mandelic acid
benzyl ester
0.40

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