Applications

Analgesics, Pharmaceutical Formulations

Phenacetin, Codeine - Alumina

	Operating Conditions Sample Dissolved in: iso-octane: methylene chloride = 1:1 Adsorbent Used: DAI Alumina Stationary Phase: 5% triethanolamine Eluent: iso-octane: methylene chloride = 1:1 Sample Volume: 1 ml Flow rate: 3 ml/min Operating Pressure: 24 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 253 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 1.75 9 2. codeine 0.5 19.5 3. phenacetin 0.05 40.5

Phenacetin, Codeine - Silica

	Operating Conditions Sample Dissolved in: methanol Adsorbent Used: DAI Silica 32-63 Stationary Phase: 25% of solution at 25% NH2 in H2O Eluent: methanol + 25% of solution of 25% NH2 in H2O Sample Volume: 1.0ml Flow rate: 12 ml/min Operating Pressure: 52 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 253 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. phenacetin 0.05 12.0 2. codeine 0.50 96.0

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