Applications

Barbituates

Pheno-, Cyclo-, Hexo-Barbital - Alumina

	Operating Conditions Sample Dissolved in: chloroform/1-chlorobutane Adsorbent Used: DAI Alumina Stationary Phase: None Eluent: chloroform: 1-chlorobutane: acetic acid = 39:60:1 Sample Volume: 1 ml Flow rate: 5 ml/min Operating Pressure: 14 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 251 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. phenobarbital 0.5 20 2. cyclobarbital 0.5 80 3. hexobarbital 0.5 130

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