Applications

Barbituates

Methyl, Hexo-, Pheno-Barbital - Silica

	Operating Conditions Sample Dissolved in: chloroform/chlorobutane/acetic acid Adsorbent Used: DAI Silica 32-63 Stationary Phase: None Eluent: chloroform: 1-chlorobutane: acetic acid = 39:60:1 Sample Volume: 1ml Flow rate: 5 ml/min Operating Pressure: 19.6 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 251 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 3.5 17.5 2. methylphenobarbital 0.5 47.5 3. hexobarbital 0.5 72.5 4. phenobarbital 0.5 150

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