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Applications Dyes, Test Mixture Azobenzene, butter yellow, Sudan Red G, indophenol blue - Silica Operating Conditions Sample Dissolved in: iso-octane/methylene chloride Adsorbent Used: DAI Silica 32-63 Stationary Phase: 15% triethanolamine Eluent: iso-octane: methylene chloride: ethanol: water = 90:10:9:1 Sample Volume: 0.5 ml Flow rate: 13 ml/min Operating Pressure: 84 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 251 nm Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 0.25 26 2. o-nitroaniline 0.25 84 3. m-nitroaniline 0.25 143 4. p-nitroaniline 0.25 338 Azobenzene, butter yellow, Sudan Red G, indophenol blue - Alumina Operating Conditions Sample Dissolved in: iso-octane/methylene chloride Adsorbent Used: DAI Alumina Stationary Phase: 10% water Eluent: iso-octane: methylene chloride = 9:1 Sample Volume: 0.15 ml Flow rate: 8 ml/min Operating Pressure: 28 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 251 nm Substances Separated Sample (mg) Retention Volume (ml) 1. azobenzene 0.6 12 2. butter yellow 0.6 32 3. sudan red G 0.6 96 4. indophenol blue 0.6 184 About Dynamic | Products | Alumina | Why Dynamic | Applications | Technical Info | FAQ/Helpdesk | Catalog | Shop Online News | Blog | Contact Us | Home
Applications
Dyes, Test Mixture
Operating Conditions
About Dynamic | Products | Alumina | Why Dynamic | Applications | Technical Info | FAQ/Helpdesk | Catalog | Shop Online News | Blog | Contact Us | Home
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