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Applications

Dyes, Test Mixture

Azobenzene, butter yellow, Sudan Red G, indophenol blue - Silica

 

Operating Conditions

Sample Dissolved in: iso-octane/methylene chloride
Adsorbent Used: DAI Silica 32-63
Stationary Phase: 15% triethanolamine
Eluent: iso-octane: methylene chloride:
ethanol: water = 90:10:9:1
Sample Volume: 0.5 ml
Flow rate: 13 ml/min
Operating Pressure: 84 psi
Column Size: 9mm x 300 mm
Temperature: 20ºC
Detection: UV detector 251 nm
Substances Separated Sample (mg) Retention Volume (ml)
1. benzene 0.25 26
2. o-nitroaniline 0.25 84
3. m-nitroaniline 0.25 143
4. p-nitroaniline 0.25 338
Azobenzene, butter yellow, Sudan Red G, indophenol blue - Alumina

 

Operating Conditions

Sample Dissolved in: iso-octane/methylene chloride
Adsorbent Used: DAI Alumina
Stationary Phase: 10% water
Eluent: iso-octane: methylene chloride = 9:1
Sample Volume: 0.15 ml
Flow rate: 8 ml/min
Operating Pressure: 28 psi
Column Size: 9mm x 300 mm
Temperature: 20ºC
Detection: UV detector 251 nm
Substances Separated Sample (mg) Retention Volume (ml)
1. azobenzene 0.6 12
2. butter yellow 0.6 32
3. sudan red G 0.6 96
4. indophenol blue 0.6 184

 

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Dynamic Adsorbents, Inc. 3280 Peachtree Corners Circle, Suite E Norcross, GA 30092  770-817-0123  Toll-Free:  1-866-314-SORB (7672) FAX:  770-455-4380 info@dynamicadsorbents.com