Applications
 

Acids, Amines, Esters, Nitrocompounds Aromatic and Plasticizers

Isomeric Phenylendiamines -  Alumina

	Operating Conditions Sample Dissolved in: iso-octane/methylene chloride Adsorbent Used: DAI Alumina Stationary Phase: water (10%) + triethanolamine (15%) Eluent: iso-octane (77) methylene chloride (12) ethanol (10) water (1) Sample Volume: 0.5 ml Flow rate: 14 ml/min Operating Pressure: 77 psi Column Size: 9mm x 500 mm Temperature: 20ºC Detection: UV detector 251 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 0.25 14 2. o-nitrophenol 0.25 126 3. m-nitrophenol 0.25 252 4. p-nitrophenol 0.25 468

Isomeric Phenylendiamines - Silica

	Operating Conditions Sample Dissolved in: benzene Adsorbent Used: DAI Silica 32-63 Stationary Phase: triethanolamine (15%) Eluent: iso-octane (70) methylene chloride (15) ethanol (14) water (1) Sample Volume: 0.5 ml Flow rate: 15 ml/min Operating Pressure: 42 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 251 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. o-nitrophenol 0.25 75 2. m-nitrophenol 0.25 142 3. p-nitrophenol 0.25 232

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