Applications

Acids or Nitrocompounds Aromatic

Isomeric Nitroaniline - Alumina

	Operating Conditions Sample Dissolved in: iso-octane/methylene chloride Adsorbent Used: DAI Alumina Stationary Phase: 5% triethanolamine + 5% water Eluent: iso-octane: methylene chloride: ethanol: water = 80:10:9:1 Sample Volume: 0.5 ml Flow rate: 13 ml/min Operating Pressure: 84 psi Column Size: 9mm x 500 mm Temperature: 20ºC Detection: UV detector 251 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 0.25 20 2. o-nitroaniline 0.25 58 3. m-nitroaniline 0.25 98 4. p-nitroaniline 0.25 234

Isomeric Nitroaniline - Silica

	Operating Conditions Sample Dissolved in: iso-octane/methylene chloride Adsorbent Used: DAI Silica 32-63 Stationary Phase: 15% triethanolamine Eluent: iso-octane: methylene chloride: ethanol: water = 90:10:9:1 Sample Volume: 0.5 ml Flow rate: 13 ml/min Operating Pressure: 84 psi Column Size: 9mm x 300 mm Temperature: 20ºC Detection: UV detector 251 nm
	Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 0.25 26 2. o-nitroaniline 0.25 84 3. m-nitroaniline 0.25 143 4. p-nitroaniline 0.25 338

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