Applications
 

Acids, Amines & Esters Aromatic

Isomeric aminobenzoic acids, phthalates - Alumina
 

	Operating Conditions Sample Dissolved in: ethyl alcohol 96% Adsorbent Used: DAI Alumina Super I Neutral Stationary Phase: 5% water Eluent: chloroform + 2% acetic acid (glacial) Sample Volume: 1.0 ml Flow rate: 7.0 ml/min Operating Pressure: 35 psi Column Size: 9mm x 300mm Temperature: 20ºC Detection: UV detector 257mm
	Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 0.44 7.0 2. o-aminobenzoic acid 0.10 42.0 3. p-aminobenzoic acid 0.10 84.0 4. m-aminobenzoic acid 0.10 210.0

Isomeric aminobenzoic acids, phthalates - Silica
 

	Operating Conditions Sample Dissolved in: ethyl alcohol (96%) Adsorbent Used: DAI Silica 32-63 Stationary Phase: 5% water Eluent: methylene chloride: ethyl alcohol (96%) = 97.5:2.5 v/v Sample Volume: 1.0 ml Flow rate: 11 ml/min Operating Pressure: 35 psi Column Size: 9mm x 300mm Temperature: 20ºC
	Substances Separated Sample (mg) Retention Volume (ml) 1. benzene 0.44 11.0 2. o-aminobenzoic acid 0.10 92.4 3. p-aminobenzoic acid 0.10 140.8 4. m-aminobenzoic acid 0.10 493.2

 

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