TLC Plates
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TLC Plates Online
Thin layer chromatography is the
initial tool for the characterization and separation
of many organic compounds. This analytical technique
is essential for the identification, separation and
purification of substances having useful biological
activity.
Dynamic Adsorbents' technology
and experience has resulted in one of the broadest
TLC-HPTLC-"S" HPTLC programs in the global
scientific community. Plates manufactured by DAI
incorporate refined silica gels, aluminas, cellulose
and PEI cellulose. The materials layered upon the
plates are offered in both normal and reverse phase.
What makes DAI TLC plates special is that we use the
best alumina and silica gels in the industry, and
have independent verification of such claims to
prove our point. Additionally we supply these same
materials in a broad variety of custom tailored
layer and plate types.
Thin layer chromatography (TLC)
is among the most useful tools for following the
progress of organic chemical reactions and for
assaying the purity of organic compounds. To this
day, TLC remains the simplest, quickest, and least
inexpensive procedure providing chemists and
separation scientists with a quick answer for
determining how many components are contained in a
mixture.
In this regard, TLC is truly a
quick, inexpensive microscale technique that can be
used to:
-
determine the number of
components in a mixture
-
verify a substance’s
identity
-
monitor the progress of a
reaction
-
determine appropriate
conditions for column chromatography
-
analyze the fractions
obtained from column chromatography
TLC plates routinely contain a
fluorescent indicator allowing TLC plates to glow
green under ultraviolet (UV) light of wavelength 254
nm. Compounds which absorb UV light will quench the
green fluorescence yielding dark purple or bluish
spots on the plate. Simply put the plate under a UV
lamp, and the compounds become visible to the naked
eye.
TLC requires only a few
nanograms (ng) of sample for a successful analysis
and can be accomplished in a matter of minutes. Like
all chromatographic methods, TLC takes advantage of
the different affinity of the analyte with the
mobile and stationary phases allowing separation of
complex mixtures of organic molecules. Since only a
few micrograms of material in solution are necessary
to observe the solute on a TLC plate this process,
unlike many other separation methods, is truly a
microscale technique.
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TLC Plates Online
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